Deriving Probes From Large‐Insert Clones by PCR Methods
| Autor: | Paul Watkins, John H. Riley, Hans Albertsen, Craig T. Basson, David J. Munroe, Andrew Thliveris |
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| Rok vydání: | 1998 |
| Předmět: |
Yeast artificial chromosome
Genetics Medical Genetic Vectors Molecular Probe Techniques Biology Polymerase Chain Reaction DNA sequencing Insert (molecular biology) law.invention chemistry.chemical_compound Alu Elements law medicine Humans Genomic library Cloning Molecular Chromosomes Artificial Yeast In Situ Hybridization Fluorescence Polymerase chain reaction Cloning medicine.diagnostic_test Chromosomes Artificial P1 Bacteriophage General Medicine Molecular biology chemistry DNA Probes DNA Fluorescence in situ hybridization |
| Zdroj: | Current Protocols in Human Genetics |
| ISSN: | 1934-8258 1934-8266 |
| DOI: | 10.1002/0471142905.hg0509s17 |
| Popis: | This unit describes several polymerase chain reaction (PCR)-based methods to obtain DNA fragments from clones with large inserts without prior knowledge of the insert DNA sequence. The protocols can be categorized into three groups: (1) methods to generate DNA fragments at random representing the entire length of the cloned insert, (2) methods to generate DNA fragments representing the extremities of an insert, and (3) methods to generate complex probes suitable for fluorescence in situ hybridization. Support protocols describe direct cloning of these PCR products and the isolation of total yeast DNA from yeast artificial chromosome (YAC) clones. |
| Databáze: | OpenAIRE |
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