Processing of the Plasmodium chabaudi chabaudi AS merozoite surface protein 1 in vivo and in vitro
| Autor: | K. Neil Brown, Alan S. Harris, Kieran P. O’Dea, Paul G. McKean |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_treatment
Molecular Sequence Data Plasmodium falciparum Protozoan Proteins Antigens Protozoan Parasitemia Cleavage (embryo) complex mixtures Plasmodium chabaudi Schizogony Mice Species Specificity In vivo Endopeptidases parasitic diseases medicine Animals Amino Acid Sequence Protein Precursors Peptide sequence Merozoite Surface Protein 1 Protease Sequence Homology Amino Acid biology General Medicine biology.organism_classification In vitro Malaria Cell biology Sequence Alignment |
| Zdroj: | Molecular and Biochemical Parasitology. 72:111-119 |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/0166-6851(95)00090-n |
| Popis: | Processing of the Plasmodium merozoite surface protein 1 (MSP-1) has been described for parasites maintained under in vitro conditions. We have now demonstrated, using CBA/Ca mice infected with Plasmodium chabaudi chabaudi AS, that MSP-1 processing also occurs in vivo. The major proteolytic cleavage sites and a processing scheme were deduced from N-terminal amino-acid sequences of the MSP-1 breakdown products. Comparison of MSP-1 processing in P. falciparum and P.c. chabaudi indicates a degree of conservation and in two cases the position of protease cleavage appears identical. Significant amounts of MSP-1 polypeptides are found in plasma during schizogony. Various aspects of MSP-1 processing including immunological and physiological reactions in the host during the critical period of schizogony can now be examined in vivo. |
| Databáze: | OpenAIRE |
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