Membrane filter method for enumerating Escherichia coli
| Autor: | E R Strickland, Alfred P. Dufour, V. J. Cabelli |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Bacteriological Techniques
Ecology Temperature Rapid urease test Fresh Water Membrane filter Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Culture Media Microbiology Fresh water Escherichia coli medicine Seawater Subculture (biology) Water Microbiology Incubation Filtration Bacteria Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 41:1152-1158 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.41.5.1152-1158.1981 |
| Popis: | A membrane filter procedure for enumerating Escherichia coli was developed and evaluated. The method quantifies E. coli within 24 h without requiring subculture and identification of isolates. It incorporates a primary selective-differential medium for gram-negative, lactose-fermenting bacteria; resuscitation of weakened organisms by incubation for 2 h at 35 degrees C before incubation at 44.5 degrees C for 18 to 22 h; and an in situ urease test to differentiate E. coli from other thermotolerant, lactose-positive organisms. The recovery of E. coli from marine, estuarine, and freshwater samples exceeded 90%. Of the presumptively positive colonies, 91% were verified as E. coli. Less than 1% of all of the verified E. coli colonies failed to react typically. |
| Databáze: | OpenAIRE |
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