A functional nuclear localization sequence in the VP1 capsid protein of coxsackievirus B3
| Autor: | Zhaohua Zhong, Xiaoning Si, Shuo Wu, Wenran Zhao, Yelu Han, Xiaoyan Zhong, Xia Zhai, Lexun Lin, Bohai Yu, Tianying Wang, Ying Qin, Lei Tong, Zhiwei Guo, Yan Wang, Fengmin Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
viruses
Recombinant Fusion Proteins Molecular Sequence Data Nuclear Localization Signals Biology Transfection Article Virology medicine Humans Amino Acid Sequence Nuclear protein Peptide sequence Coxsackievirus B3 Cell Nucleus Viral Structural Proteins Sequence Homology Amino Acid Cell Cycle RNA virus diseases Cell cycle biochemical phenomena metabolism and nutrition Molecular biology VP1 capsid protein Enterovirus B Human Nuclear localization medicine.anatomical_structure Capsid Amino Acid Substitution Cytoplasm Mutagenesis Site-Directed Nucleus Nuclear localization sequence HeLa Cells |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2012.08.040 |
| Popis: | The capsid proteins of some RNA viruses can translocate to the nucleus and interfere with cellular phenotypes. In this study we found that the VP1 capsid protein of coxsackievirus B3 (CVB3) was dominantly localized in the nucleus of the cells transfected with VP1-expressing plasmid. The VP1 nuclear localization also occurred in the cells infected with CVB3. Truncation analysis indicated that the VP1 nuclear localization sequence located near the C-terminal. The substitution of His220 with threonine completely abolished its translocation. The VP1 proteins of other CVB types might have the nuclear localization potential because this region was highly conserved. Moreover, the VP1 nuclear localization induced cell cycle deregulation, including a prolonged S phase and shortened G2-M phase. Besides these findings, we also found a domain between Ala72 and Phe106 that caused the VP1 truncates dotted distributed in the cytoplasm. Our results suggest a new pathogenic mechanism of CVB. Highlights ► The VP1 protein of coxsackievirus B3 can specifically localize in the nucleus. ► The nuclear localization signal of coxsackievirus B3 VP1 protein locates near its C-terminal. ► The VP1 nuclear localization of coxsackievirus B3 can deregulate cell cycle. ► There is a domain in the VP1 that determines it dotted distributed in the cytoplasm. |
| Databáze: | OpenAIRE |
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