Acceptor specificity of cellobiose phosphorylase from Cellvibrio gilvus: synthesis of three branched trisaccharides
| Autor: | Kiyoshi Hayashi, Ann E. Percy, Hiroshi Ono |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Magnetic Resonance Spectroscopy
Stereochemistry Molecular Sequence Data Substituent Spectrometry Mass Fast Atom Bombardment Disaccharides Biochemistry Analytical Chemistry Substrate Specificity chemistry.chemical_compound Glucuronamide Cellobiose phosphorylase Gentiobiose Organic chemistry Monosaccharide Melibiose chemistry.chemical_classification Gram-Negative Aerobic Bacteria Organic Chemistry Glucosephosphates General Medicine Isomaltose Acceptor chemistry Carbohydrate Sequence Glucosyltransferases Trisaccharides |
| Zdroj: | Carbohydrate research. 308(3-4) |
| ISSN: | 0008-6215 |
| Popis: | Cellobiose phosphorylase from Cellvibrio gilvus was examined for its acceptor specificity in the synthetic reaction with glucose-1-phosphate, using substrates in which the C-6 substituent of D-Glc had been altered. A range of disaccharides were also tested for acceptor specificity but only those with (1--6)-linkages were successful acceptors. Melibiose, gentiobiose, isomaltose and also the monosaccharide glucuronamide were found to react with cellobiose phosphorylase and glucose-1-phosphate giving beta-D-Glcp-(1--4)-[alpha-D-Galp-(1--6)]-D-Glcp, beta-D-Glcp-(1--4)-[beta-D-Glcp-(1--6)]-D-Glcp, beta-D-Glcp-(1--4)-[alpha-D-Glcp-(1--6)]-D-Glcp and beta-D-Glcp-(1--4)-D-GlcUNp, respectively. These products were purified using a range of chromatographic methods and characterised by NMR and FAB-MS. This is the first time cellobiose phosphorylase has been shown to synthesise trisaccharides. |
| Databáze: | OpenAIRE |
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