Islet-on-a-chip: Biomimetic micropillar-based microfluidic system for three-dimensional pancreatic islet cell culture
| Autor: | Kamil Zukowski, Zbigniew Brzozka, Agnieszka Dobrzyn, Elzbieta Jastrzebska, Justyna Janikiewicz, Patrycja Sokolowska |
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| Rok vydání: | 2021 |
| Předmět: |
Confocal
Cell Microfluidics Biomedical Engineering Biophysics Cell Culture Techniques 02 engineering and technology Biosensing Techniques 01 natural sciences Islets of Langerhans In vivo Biomimetics Lab-On-A-Chip Devices Electrochemistry medicine Humans Insulin geography geography.geographical_feature_category Microwell Plate Chemistry Pancreatic islets 010401 analytical chemistry Glucagon secretion General Medicine 021001 nanoscience & nanotechnology Islet Cell aggregation 0104 chemical sciences Cell biology medicine.anatomical_structure Glucose Diabetes Mellitus Type 2 0210 nano-technology Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 183 |
| ISSN: | 1873-4235 |
| Popis: | Type 2 diabetes is currently one of the most common metabolic diseases, affecting all ages worldwide. As the incidence of type 2 diabetes increases, a growing number of studies focus on islets of Langerhans. A three-dimensional research model that maps islet morphology and maintains hormonal balance in vivo is still needed. In this work, we present an Islet-on-a-chip system, specifically a micropillar-based microfluidic platform for three-dimensional pancreatic islet cell culture and analysis. The microfluidic system consisted of two culture chambers that were equipped with 15 circular microtraps each, which were built with seven round micropillars each. Micropillars in the structure of microtraps supported cell aggregation by limiting the growth surface and minimizing wall shear stress, thereby ensuring proper medium diffusion and optimal culture conditions for cell aggregates. Our system is compatible with microwell plate readers and confocal laser scanning microscopes. Because of optimization of the immunostaining method, the appropriate cell distribution and high viability and proliferation up to 72 h of culture were confirmed. Enzyme-linked immunosorbent assays were performed to measure insulin and glucagon secretion after stimulation with different glucose concentrations. To our knowledge, this is the first Lab-on-a-chip system which enables the formation and three-dimensional culture of cell aggregates composed of commercially available α and β pancreatic islet cells. The specific composition and arrangement of cells in the obtained model corresponds to the arrangement of the cells in rodent pancreatic islets in vivo. This Islet-on-a-chip system may be utilized to test pathogenic effectors and future therapeutic agents. |
| Databáze: | OpenAIRE |
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