A simple specific radioenzymatic assay for the simultaneous measurement of picogram quantities of norepinephrine, epinephrine, and dopamine in plasma and tissues
| Autor: | M. Nasir Hussain, Michael J. Sole |
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| Rok vydání: | 1977 |
| Předmět: |
Chromatography
Catechol-O-methyl transferase Epinephrine Chemistry Silica gel Dopamine Microchemistry Liquid scintillation counting Catechol O-Methyltransferase Tritium Biochemistry Rats Norepinephrine (medication) Norepinephrine chemistry.chemical_compound Liver Isotope Labeling medicine Catecholamine Animals medicine.drug |
| Zdroj: | Biochemical Medicine. 18:301-307 |
| ISSN: | 0006-2944 |
| DOI: | 10.1016/0006-2944(77)90064-3 |
| Popis: | A sensitive simple radiometric method for the simultaneous measurement of norepinephrine, epinephrine, and dopamine is described. Fifty microliters of plasma or tissue extract was incubated with catechol-O-methyl transferase and tritiated S-adenosylmethionine. The catecholamines were converted to their O-methylated tritiated derivatives. These derivatives were purified by solvent extraction and were isolated by one-dimensional silica gel thin-layer chromatography. The spots containing the O-methylated derivatives were scraped directly into vials and were counted by liquid scintillation. Approximately one picogram of each catecholamine could be measured with an interassay coefficient of variation of 4.3, 8.9, and 13.2% for norepinephrine, epinephrine, and dopamine, respectively. No cross-reactivity was noted for several compounds related to these catecholamines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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