Participation of Hsp70 and Hsp90α Heat Shock Proteins in Stress Response in the Course of Type 1 Diabetes Mellitus
Autor: | S. B. Parfenyuk, Olga V. Glushkova, Sergey M Lunin, T. V. Novoselova, Elena G Novoselova, E. E. Fesenko, Maxim O. Khrenov |
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Rok vydání: | 2020 |
Předmět: |
medicine.medical_specialty
Antioxidant endocrine system diseases medicine.medical_treatment General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stress Physiological Insulin-Secreting Cells Internal medicine Heat shock protein Diabetes mellitus Alloxan medicine Animals HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins Mice Inbred BALB C 0303 health sciences Type 1 diabetes General Immunology and Microbiology Chemistry 030302 biochemistry & molecular biology General Medicine medicine.disease In vitro Rats Hsp70 Diabetes Mellitus Type 1 Cytokine Endocrinology General Agricultural and Biological Sciences Spleen 030217 neurology & neurosurgery Peroxiredoxin VI |
Zdroj: | Doklady Biological Sciences. 493:124-127 |
ISSN: | 1608-3105 0012-4966 |
DOI: | 10.1134/s0012496620040079 |
Popis: | The role of two heat shock proteins, Hsp70 and Hsp90α, on stress response in mice with severe diabetes mellitus induced by a high dose of alloxan (500 mg/kg body weight), as well as in RIN-m5F β cells cultured in the presence of cytokines (IL-1 and TNF-α) was studied. Our results showed that severe type 1 diabetes mellitus (T1D) caused a higher expression of Hsp90α, but not Hsp70. Moreover, injections of the peroxiredoxin 6 antioxidant enzyme (PRDX6) did not affect the expression of these chaperones. Conversely, pro-inflammatory cytokines added to β-cells caused a significant increase in the expression of Hsp90α and, substantially, Hsp70. Moreover, cells cultivated in the presence of PRDX6 were more susceptible to the cytokine effect. Thus, in the course of severe alloxan-induced T1D, no protective role of the heat shock proteins, was revealed, and their expression level was not increased by PRDX6. At the same time the protective potential of these proteins was shown in vitro with the use of RIN-m5F β cells. Thus, the system of heat shock proteins was unable to prevent the devastating effects of severe T1D accompanied by high animal mortality. |
Databáze: | OpenAIRE |
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