Multispectral photoacoustic microscopy based on an optical–acoustic objective
| Autor: | Joseph P. Kilroy, Tianxiong Wang, Bo Ning, Rui Cao, Song Hu, John A. Hossack |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
media_common.quotation_subject Confocal Multispectral image lcsh:QC221-246 Photoacoustic microscopy medicine.disease_cause law.invention Multispectral imaging Optics law Chromatic aberration medicine Contrast (vision) lcsh:QC350-467 Radiology Nuclear Medicine and imaging Letter to the Editor media_common Optical–acoustic objective business.industry Lipid Laser Atomic and Molecular Physics and Optics lcsh:QC1-999 lcsh:Acoustics. Sound Ultrasonic sensor Blood vessel sense organs Cell nucleus business Ultraviolet lcsh:Physics lcsh:Optics. Light Biomedical engineering |
| Zdroj: | Photoacoustics Photoacoustics, Vol 3, Iss 2, Pp 55-59 (2015) |
| ISSN: | 2213-5979 |
| Popis: | We have developed reflection-mode multispectral photoacoustic microscopy (PAM) based on a novel optical–acoustic objective that integrates a customized ultrasonic transducer and a commercial reflective microscope objective into one solid piece. This technical innovation provides zero chromatic aberration and convenient confocal alignment of the optical excitation and acoustic detection. With a wavelength-tunable optical-parametric-oscillator laser, we have demonstrated multispectral PAM over an ultrabroad spectral range of 270–1300nm. A near-constant lateral resolution of ∼2.8μm is achieved experimentally. Capitalizing on the consistent performance over the ultraviolet, visible, and near-infrared range, multispectral PAM enables label-free concurrent imaging of cell nucleus (DNA/RNA contrast at 270nm), blood vessel (hemoglobin contrast at 532nm), and sebaceous gland (lipid contrast at 1260nm) at the same spatial scale in a living mouse ear. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|