L-methionine degradation pathway in Kluyveromyces lactis: identification and functional analysis of the genes encoding L-methionine aminotransferase
| Autor: | Pascal Bonnarme, Cécile Neuvéglise, Serge Casaregola, Dafni-Maria Kagkli, Timothy M. Cogan |
|---|---|
| Přispěvatelé: | Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Moorepark Food Research Centre, Teagasc - The Agriculture and Food Development Authority (Teagasc), Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), D. Kagkli bénéficie Marie Curie Fellowship (YETI program QLK3_CT_2000_60055) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Genetics and Molecular Biology
MESH: Transformation Genetic medicine.disease_cause Applied Microbiology and Biotechnology MESH: Kluyveromyces Transformation 03 medical and health sciences chemistry.chemical_compound Kluyveromyces Plasmid Transformation Genetic Methionine Genetic MESH: Plasmids MESH: Sulfur Compounds MESH: Transaminases medicine Escherichia coli Sulfhydryl Compounds MESH: Sulfhydryl Compounds Gene Transaminases 030304 developmental biology Kluyveromyces lactis 0303 health sciences Ecology biology Sulfur Compounds 030306 microbiology MESH: Escherichia coli [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Yeast Metabolic pathway chemistry Biochemistry MESH: Methionine MESH: Volatilization Volatilization Food Science Biotechnology Plasmids |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (5), pp.3330-5. ⟨10.1128/AEM.72.5.3330-3335.2006⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.72.5.3330-3335.2006⟩ |
| Popis: | Kluyveromyces lactis is one of the cheese-ripening yeasts and is believed to contribute to the formation of volatile sulfur compounds (VSCs) through degradation of l -methionine. l -Methionine aminotransferase is potentially involved in the pathway that results in the production of methanethiol, a common precursor of VSCs. Even though this pathway has been studied previously, the genes involved have never been studied. In this study, on the basis of sequence homology, all the putative aminotransferase-encoding genes from K. lactis were cloned in an overproducing vector, pCXJ10, and their effects on the production of VSCs were analyzed. Two genes, Kl ARO8.1 and Kl ARO8.2 , were found to be responsible for l -methionine aminotransferase activity. Transformants carrying these genes cloned in the pCXJ10 vector produced threefold-larger amounts of VSCs than the transformant containing the plasmid without any insert or other related putative aminotransferases produced. |
| Databáze: | OpenAIRE |
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