Antifungal Activity of Essential Oil Compounds (Geraniol and Citral) and Inhibitory Mechanisms on Grain Pathogens (Aspergillus flavus and Aspergillus ochraceus)
| Autor: | Ya-Jie Tang, Tang Xi, Ye-Lin Shao, Wen-Wen Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Preservative 030106 microbiology Pharmaceutical Science Aspergillus flavus Citral Analytical Chemistry law.invention lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound lcsh:Organic chemistry law Drug Discovery Grain quality Food science Physical and Theoretical Chemistry geraniol citral Essential oil reactive oxygen species Aspergillus modified atmosphere packaging biology antifungal activity Organic Chemistry food and beverages biology.organism_classification relative electric conductivity 030104 developmental biology chemistry Chemistry (miscellaneous) gene expression Molecular Medicine Aspergillus ochraceus Geraniol |
| Zdroj: | Molecules Volume 23 Issue 9 Molecules, Vol 23, Iss 9, p 2108 (2018) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules23092108 |
| Popis: | The grain contamination by Aspergillus spp. has been a serious issue. This study exhibited the excellent antifungal effects of the essential oil compounds (EOCs) geraniol and citral against common grain pathogens (A. flavus and A. ochraceus) in vitro and in situ. The inhibitory mechanisms were also evaluated from the perspective of cell membrane permeability, reactive oxygen species (ROS) generation, and Aspergillus spp. growth-related gene expression. Meanwhile, the combined effects of EOCs in the vapor phase and modified atmosphere packaging (MAP) were examined to find an alternative preservation method for controlling Aspergillus spp. The results indicated that citral exhibited the antifungal activity mainly by downregulating the sporulation- and growth-related genes for both pathogens. Geraniol displayed inhibitory effectiveness against A. flavus predominantly by inducing the intracellular ROS accumulation and showed toxicity against A. ochraceus principally by changing cell membrane permeability. Furthermore, the synthetic effects of EOCs and MAP (75% CO2 and 25% N2) induced better grain quality than the current commercial fumigant AlP. These findings reveal that EOCs have potential to be a novel grain preservative for further application. |
| Databáze: | OpenAIRE |
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