Differential regulation of steroidogenic enzymes during differentiation optimizes testosterone production by adult rat Leydig cells
| Autor: | Li-Xin Shan, Matthew P. Hardy, David M. Phillips, C W Bardin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Aging endocrine system medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases medicine.drug_class Cellular differentiation Biology Rats Sprague-Dawley 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) Endocrinology Internal medicine Testis medicine Animals Testosterone Cholesterol Side-Chain Cleavage Enzyme RNA Messenger Progenitor cell Leydig cell Endoplasmic reticulum Cholesterol side-chain cleavage enzyme Leydig Cells Steroid 17-alpha-Hydroxylase Cell Differentiation Leydig cell differentiation Luteinizing Hormone Androgen Rats medicine.anatomical_structure Gene Expression Regulation Female |
| Zdroj: | Endocrinology. 133:2277-2283 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | The postnatal differentiation of rat Leydig cells may be subdivided into three stages based on morphology and steroid production. The purpose of this study was to clarify the developmental mechanisms underlying increased testosterone production by measuring steady state levels of the mRNAs for three steroidogenic enzymes in isolated Leydig cells at each stage of differentiation. These include Leydig cell progenitors on day 21, immature Leydig cells on day 35, and adult Leydig cells on day 90. The steroidogenic enzymes were 1) cholesterol side-chain cleavage enzyme (CSCC), 2) 17 alpha-hydroxylase (P450-17 alpha), and 3) 3 alpha-hydroxysteroid dehydrogenase (3 alpha HSD). We report that levels of CSCC and P450-17 alpha mRNAs increase, whereas 3 alpha HSD mRNA levels decline during the course of Leydig cell differentiation. The levels of 3 alpha HSD mRNA were high in progenitor Leydig cells that appeared to contain little smooth endoplasmic reticulum and decreased in cells as smooth endoplasmic reticulum developed and other enzyme mRNAs increased. These observations suggest that the factors that regulate 3 alpha HSD mRNA levels are startlingly different from those that regulate the mRNA levels of CSCC and P450-17 alpha. We conclude that the progressive increase in the capacity of differentiating Leydig cells to produce testosterone can be explained in part by an increase in the activity of enzymes that synthesize testosterone (CSCC and P450-17 alpha) and a decrease in the activity of an enzyme that metabolizes testosterone and its precursors (3 alpha HSD). |
| Databáze: | OpenAIRE |
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