In vitro activity of antimicrobial agents against Legionella isolated from environmental water systems: first results from Turkey

Autor: Hande Arslan, Müge Demirbilek, Funda Timurkaynak, Haluk Erdoğan, Fusun Can
Rok vydání: 2010
Předmět:
Zdroj: Environmental Monitoring and Assessment. 171:487-491
ISSN: 1573-2959
0167-6369
Popis: We evaluated the in vitro activity of antimicrobial agents against Legionella obtained from hotel and hospital water systems in three different regions of Turkey. Sixty-five Legionella strains (Legionella pneumophila serogroup 6 [n = 32], L. pneumophila serogroup 1 [n = 27], L. pneumophila serogroup 3 [n = 2], and Legionella spp. [n = 4]) were tested against levofloxacin, ciprofloxacin, clarithromycin, azithromycin, and rifampicin. The minimum inhibitory concentration (MIC) values of each antimicrobial agent for these strains was determined by the microdilution method using buffered yeast extract medium supplemented with 0.1% ketoglutarate broth. L. pneumophila ATCC 33152, Staphylococcus aureus ATCC 29213, and Escherichia coli ATCC 25922 were used as controls. Minimum inhibitory concentration values were in the following ranges: clarithromycin 0.001–0.5 mg/L, azithromycin 0.001–0.5 mg/L, levofloxacin 0.001–0.5 mg/L, ciprofloxacin 0.001–0.125 mg/L, and rifampicin 0.001– 0.5 mg/L. The MIC90 for rifampicin, levofloxacin, ciprofloxacin, azithromycin, and clarithromycin were 0.015, 0.125, 0.06, 0.125, and 0.06 mg/L, respectively. To the best of our knowledge, this is the first study to determine in vitro activities of antimicrobial agents against Legionella species in Turkey. Rifampicin had the lowest MIC90 value. It would seem that azithromycin and clarithromycin exhibit good activity as well as levofloxacin and ciprofloxacin against Legionella isolated from environmental water systems in Turkey.
Databáze: OpenAIRE
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