6,7-di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity
| Autor: | Da-Long Zhao, Jian-Gong Shi, Hai-Bo Zhu, Sheng Lin, Li-bo Zou |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
SH-SY5Y Cell Survival Dopamine Blotting Western Apoptosis Caspase 3 Pharmacology Neuroblastoma chemistry.chemical_compound Glucosides Cell Line Tumor Internal medicine medicine Humans Neurotoxin RNA Messenger Umbelliferones Cytotoxicity bcl-2-Associated X Protein Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug Molecular Structure biology Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase Superoxide Cytochrome c Glutathione Flow Cytometry Endocrinology Fraxinus Proto-Oncogene Proteins c-bcl-2 chemistry Plant Bark biology.protein Tumor Suppressor Protein p53 Reactive Oxygen Species |
| Zdroj: | European Journal of Pharmacology. 580:329-338 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2007.11.057 |
| Popis: | Dopamine, as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species levels and apoptotic activity. In this study, we examined the effect of 6,7-di-O-glucopyranosyl-esculetin, which was extracted from Fraxinus sieboldiana bloom, on dopamine-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of 6,7-di-O-glucopyranosyl-esculetin (0.1, 1 and 10 microM) on dopamine-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing reactive oxygen species levels, and its anti-apoptotic effect via protecting mitochondrion membrane potential (Delta Psi m), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating p53, Bax and Bcl-2 expression. In addition, 6,7-di-O-glucopyranosyl-esculetin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that 6,7-di-O-glucopyranosyl-esculetin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease. |
| Databáze: | OpenAIRE |
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