Long-Term Local Injection of RAGE-Aptamer Suppresses the Growth of Malignant Melanoma in Nude Mice
Autor: | Nobutaka Nakamura, Sho-ichi Yamagishi, Yuri Nishino, Takanori Matsui, Yuichiro Higashimoto, Ami Sotokawauchi |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
CD31 Article Subject endocrine system diseases medicine.disease_cause lcsh:RC254-282 RAGE (receptor) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine cardiovascular diseases Receptor business.industry Nitrotyrosine Monocyte Melanoma nutritional and metabolic diseases lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Vascular endothelial growth factor 030104 developmental biology medicine.anatomical_structure Oncology chemistry 030220 oncology & carcinogenesis cardiovascular system Cancer research business human activities Oxidative stress Research Article |
Zdroj: | Journal of Oncology, Vol 2019 (2019) Journal of Oncology |
ISSN: | 1687-8469 1687-8450 |
DOI: | 10.1155/2019/7387601 |
Popis: | Accumulating evidence has suggested the pathological role of advanced glycation end products (AGEs) and their receptor RAGE axis in aging-associated disorders, including cancers. In this study, we examined the effects of local injection of RAGE-aptamer adjacent to the tumor on G361 melanoma growth in nude mice. We further investigated the effects of RAGE-aptamer on oxidative stress generation, RAGE, vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) gene expression inNε-(carboxymethyl)lysine (CML)-exposed G361 melanoma cellsin vitro. Local injection of RAGE-aptamer adjacent to the tumor dramatically decreased the growth of G361 melanoma in nude mice, which was associated with reduced expression of CML, RAGE, nitrotyrosine, VEGF, CD31, and von Willebrand factor, markers of endothelial cells in G361 tumors. Furthermore, RAGE-aptamer inhibited the binding of CML to V-domain of RAGE and blocked the CML-induced increases in oxidative stress generation, RAGE, VEGF, and MCP-1 mRNA levels in G361 melanoma cells. Our present findings suggest that long-term local injection of RAGE-aptamer adjacent to the tumor could inhibit melanoma growth in nude mice partly by suppressing tumor angiogenesis via blockade of the CML-RAGE interaction. Local injection of RAGE-aptamer may be a feasible therapeutic tool for the treatment of malignant melanoma. |
Databáze: | OpenAIRE |
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