Merit of quinacrine in the decrease of ingested sulfite-induced toxic action in rat brain
| Autor: | Berna Sozen, Gamze Tanriover, Narin Derin, Piraye Yargicoglu-Akkiraz, Aysel Agar, Ozlem Ozsoy, Goksun Basaranlar, Nihal Ozturk, Ceren Kencebay, Dijle Kipmen-Korgun |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
DNA damage medicine.medical_treatment Intraperitoneal injection Apoptosis Pharmacology Toxicology medicine.disease_cause Thiobarbituric Acid Reactive Substances Lipid peroxidation chemistry.chemical_compound medicine TBARS Animals Sulfites Rats Wistar Phospholipases A2 Secretory Evoked Potentials TUNEL assay Caspase 3 Brain General Medicine Sodium metabisulfite Rats chemistry Biochemistry Quinacrine Lipid Peroxidation Sulfonic Acids Oxidative stress Food Science |
| Zdroj: | Food and Chemical Toxicology. 52:129-136 |
| ISSN: | 0278-6915 |
| Popis: | We aimed at investigating the effects of sulfite-induced lipid peroxidation and apoptosis mediated by secretory phospholipase A2 (sPLA2) on somatosensory evoked potentials (SEP) alterations in rats. Thirty male albino Wistar rats were randomized into three experimental groups as follows; control (C), sodium metabisulfite treated (S), sodium metabisulfite+quinacrine treated (SQ). Sodium metabisulfite (100 mg/kg/day) was given by gastric gavage for 5 weeks and 10 mg/kg/day quinacrine was applied as a single dose of intraperitoneal injection for the same period. The latencies of SEP components were significantly prolonged in the S group and returned to control levels following quinacrine administration. Plasma-S-sulfonate level was increased in S and SQ groups. TBARS levels in the S group were significantly higher than those detected in controls. Quinacrine significantly decreased brain TBARS levels in the SQ group compared with the S group. Quinacrine treatment did not have an effect on the increased sPLA2 level of the sulfite administered group. Immunohistochemistry showed that sulfite caused an increase in caspase-3 and TUNEL positive cells, restored to control levels via quinacrine administration. This study showed that sPLA2 might play a role in ingested sulfite-induced SEP alterations, oxidative stress, apoptotic cell death and DNA damage in the brain. |
| Databáze: | OpenAIRE |
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