Huaier shows anti‐cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF‐1α pathway
| Autor: | Shuguang Zhang, Keyan Chen, Wenya Li, Xiangli Liu, Lidan Liu, Lei Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Lung Neoplasms Cell Survival Antineoplastic Agents Complex Mixtures NSCLC PI3K Small hairpin RNA 03 medical and health sciences Mice 0302 clinical medicine In vivo HIF‐1α pathway Cell Line Tumor energy metabolism medicine Animals Humans Huaier Viability assay Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation A549 cell Trametes Cell growth Chemistry AKT Cancer Cell Biology Original Articles medicine.disease Hypoxia-Inducible Factor 1 alpha Subunit Xenograft Model Antitumor Assays Disease Models Animal 030104 developmental biology Glucose 030220 oncology & carcinogenesis Cancer research Molecular Medicine Original Article Phosphatidylinositol 3-Kinase Glycolysis Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Huaier has been verified to have anti‐cancer effects on many tumours. However, little information is available about the effects of Huaier on non‐small cell lung cancer (NSCLC). We sought to probe the anti‐cancer effects and related mechanisms of Huaier on lung cancer. A549 cells were pre‐treated with 2, 4 and 8 mg/mL Huaier at different time points. Thereafter, cell viability was analysed by CCK‐8 and the migration and invasion were detected by Scratch test and Transwell chamber migration assay. Moreover, ELISA, Western blot, shRNA transfection and RT‐PCR were conducted to discover the related gene and protein expressions of energy metabolism and phosphatidylinositol 3‐kinase (PI3K)/AKT/hypoxia‐inducible factor 1α (HIF‐1α) pathway. Furthermore, tumour xenografts were accomplished to inspect the anti‐cancer effects of Huaier. Our consequences suggested that Huaier considerably repressed cell viability and migration in a dose‐dependent way. In addition, Huaier statistically suppressed glycolysis, glucose transport and lactic acid (LA) accumulation. Besides, we detected that Huaier could inactivate the PI3K/AKT/HIF‐1α pathway. The in vivo data confirmed that Huaier obviously decreased tumour volume and tumour growth, reduced the glycolysis, glucose transport and HIF‐1α expression in the tumour‐bearing tissues. Our results suggested Huaier revealed anti‐tumour effects in both in vivo and in vitro possibly through PI3K/AKT/HIF‐1α pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text