GRDN-1/Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans
| Autor: | Piali Sengupta, Inna V. Nechipurenko, Sofia Lavrentyeva |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Sensory Receptor Cells
Neurogenesis Kinesins Cell Cycle Proteins Dendrite Biology Article Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine medicine Animals Basal body Cilia Caenorhabditis elegans Caenorhabditis elegans Proteins Wnt Signaling Pathway Molecular Biology Glycoproteins 030304 developmental biology 0303 health sciences Cilium Microfilament Proteins Dendrites Cell Biology Cadherins Axons Basal Bodies Sensory neuron Cell biology Dendrite morphogenesis medicine.anatomical_structure Gene Knockdown Techniques Mutation Sensory dendrite Dendrite extension CRISPR-Cas Systems Transduction (physiology) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dev Biol |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2020.12.022 |
| Popis: | Primary cilia are located at the dendritic tips of sensory neurons and house the molecular machinery necessary for detection and transduction of sensory stimuli. The mechanisms that coordinate dendrite extension with cilium position during sensory neuron development are not well understood. Here, we show that GRDN-1, the Caenorhabditis elegans ortholog of the highly conserved scaffold and signaling protein Girdin/GIV, regulates both cilium position and dendrite extension in the postembryonic AQR and PQR gas-sensing neurons. Mutations in grdn-1 disrupt dendrite outgrowth and mislocalize cilia to the soma or proximal axonal segments in AQR, and to a lesser extent, in PQR. GRDN-1 is localized to the basal body and regulates localization of HMR-1/Cadherin to the distal AQR dendrite. However, knockdown of HMR-1 and/or loss of SAX-7/LICAM, molecules previously implicated in sensory dendrite development in C. elegans, do not alter AQR dendrite morphology or cilium position. We find that GRDN-1 localization in AQR is regulated by UNC-116/Kinesin-1, and that correspondingly, unc-116 mutants exhibit severe AQR dendrite outgrowth and cilium positioning defects. In contrast, GRDN-1 and cilium localization in PQR is modulated by LIN-44/Wnt signaling. Together, these findings identify upstream regulators of GRDN-1, and describe new cell-specific roles for this multifunctional protein in sensory neuron development. |
| Databáze: | OpenAIRE |
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