The Role of Efflux Pumps and Environmental pH in Bacterial Multidrug Resistance
| Autor: | Gabriella Spengler, Leonard Amaral, József Molnár, Márta Nové, Annamária Kincses |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Bacterial growth medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Drug Resistance Multiple Bacterial Escherichia coli medicine Gene Pharmacology Escherichia coli K12 biology Chemistry Escherichia coli Proteins Membrane Transport Proteins Gene Expression Regulation Bacterial Hydrogen-Ion Concentration biology.organism_classification SOXS Multiple drug resistance 030220 oncology & carcinogenesis Efflux Multidrug Resistance-Associated Proteins Bacteria Research Article |
| Zdroj: | In Vivo. 34:65-71 |
| ISSN: | 1791-7549 0258-851X |
| DOI: | 10.21873/invivo.11746 |
| Popis: | Background/Aim: One of the most studied bacterial resistance mechanisms is the resistance related to multidrug efflux pumps. In our study the pump activity of the Escherichia coli K-12 AG100 strain expressing the AcrAB-TolC pump system was investigated at pH 7 and pH 5 in the presence of the efflux pump inhibitor (EPI) promethazine (PMZ). Materials and Methods: The EPI activity was assessed by real-time fluorimetry. The influence of PMZ treatment on the relative expression of the pump genes acrA, acrB and their regulators marA, marB, marR, the stress genes soxS, rob, as well as the bacterial growth control genes ftsI, and sdiA were determined by RT-qPCR. Results: The EPI activity of PMZ was more effective at neutral pH. The PMZ treatment induced a significant stress response in the bacterium at acidic pH by the up-regulation of genes. Conclusion: The genetic system that regulates the activity of the main efflux pump is pH-dependent. |
| Databáze: | OpenAIRE |
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