[8]-Gingerol inhibits melanogenesis in murine melanoma cells through down-regulation of the MAPK and PKA signal pathways
| Autor: | Tsong-Min Chang, Yin-Chun Chou, Chia-Yin Wu, Huey-Chun Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
Melanogenesis Cell Survival MAP Kinase Signaling System Tyrosinase Catechols Melanoma Experimental Biophysics Down-Regulation Biology Biochemistry Gene Expression Regulation Enzymologic Melanin Mice Cell Line Tumor medicine Animals Protein kinase A [8]-Gingerol Melanoma Molecular Biology Melanins chemistry.chemical_classification Microphthalmia-Associated Transcription Factor Reactive oxygen species Monophenol Monooxygenase Kinase Cell Biology Cyclic AMP-Dependent Protein Kinases Intramolecular Oxidoreductases chemistry Mechanism of action Fatty Alcohols Signal transduction medicine.symptom Reactive Oxygen Species Receptor Melanocortin Type 1 Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 438:375-381 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.07.079 |
| Popis: | [8]-Gingerol is an active component of Zinger and shows several pharmacological activities, such as antipyretic and anti-inflammation characteristics. To identify a potential skin-whitening agent, the inhibitory effects of [8]-gingerol on melanogenesis and its mechanism of action were investigated. In the present study, the effects of [8]-gingerol on mushroom tyrosinase, tyrosinase activity and melanin content were determined spectrophotometrically; the expression of melanogenesis-related proteins in B16F10 and B16F1 melanoma cells were determined by Western blotting. Furthermore, the possible signaling pathways involved in [8]-gingerol-mediated depigmentation were also investigated using specific inhibitors. The results revealed that [8]-gingerol (5–100μM) effectively suppressed intracellular tyrosinase activity and decreased the amount of melanin in B16F10 and B16F1 cells. In addition, [8]-gingerol also effectively decreased intracellular reactive species (RS) and reactive oxygen species (ROS) levels at the same dose range. Our results indicated that [8]-gingerol inhibited melanogenesis in B16F10 and B16F1 cells by down-regulation of both mitogen-activated protein kinases (MAPK) and protein kinase A (PKA) signaling pathways or through its antioxidant properties. Hence, [8]-gingerol could be used as an effective skin-whitening agent. |
| Databáze: | OpenAIRE |
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