Naringin protects human adipose-derived mesenchymal stem cells against hydrogen peroxide-induced inhibition of osteogenic differentiation
| Autor: | Yu-ge Zhang, Xiu-Mei Wang, Yuan-Min Zhang, Long-Fei Ma, Lei Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Cell Survival Cellular differentiation Toxicology medicine.disease_cause chemistry.chemical_compound Osteogenesis Internal medicine medicine Humans Wnt Signaling Pathway Naringin Osteoblasts Mesenchymal stem cell Wnt signaling pathway Cell Differentiation Mesenchymal Stem Cells Hydrogen Peroxide General Medicine Cytoprotection Cell biology RUNX2 Oxidative Stress Endocrinology Adipose Tissue chemistry Flavanones Alkaline phosphatase Oxidative stress |
| Zdroj: | Chemico-Biological Interactions. 242:255-261 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/j.cbi.2015.10.010 |
| Popis: | Extensive evidence indicates that oxidative stress plays a pivotal role in the development of osteoporosis. We show that naringin, a natural antioxidant and anti-inflammatory compound, effectively protects human adipose-derived mesenchymal stem cells (hADMSCs) against hydrogen peroxide (H2O2)-induced inhibition of osteogenic differentiation. Naringin increased viability of hAMDSCs and attenuated H2O2-induced cytotoxicity. Naringin also reversed H2O2-induced oxidative stress. Oxidative stress induced by H2O2 inhibits osteogenic differentiation by decreasing alkaline phosphatase (ALP) activity, calcium content and mRNA expression levels of osteogenesis marker genes RUNX2 and OSX in hADMSCs. However, addition of naringin leads to a significant recovery, suggesting the protective effects of naringin against H2O2-induced inhibition of osteogenic differentiation. Furthermore, the H2O2-induced decrease of protein expressions of β-catenin and clyclin D1, two important transcriptional regulators of Wnt-signaling, was successfully rescued by naringin treatment. Also, in the presence of Wnt inhibitor DKK-1, naringin is no longer effective in stimulating ALP activity, increasing calcium content and mRNA expression levels of RUNX2 and OSX in H2O2-exposed hADMSCs. These data clearly demonstrates that naringin protects hADMSCs against oxidative stress-induced inhibition of osteogenic differentiation, which may involve Wnt signaling pathway. Our work suggests that naringin may be a useful addition to the treatment armamentarium for osteoporosis and activation of Wnt signaling may represent attractive therapeutic strategy for the treatment of degenerative disease of bone tissue. |
| Databáze: | OpenAIRE |
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