On-line screening and identification of free radical scavenging compounds in Angelica dahurica fermented with Eurotium cristatum using an HPLC-PDA-Triple-TOF-MS/MS-ABTS system
| Autor: | Siduo Zhou, Mingsheng Dong, Lu Huang, Yan-Fei Lin, Hai-Yan Xia, Xiao Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Online Systems 01 natural sciences High-performance liquid chromatography Indole Alkaloids Analytical Chemistry chemistry.chemical_compound Bioreactors 0404 agricultural biotechnology Tandem Mass Spectrometry Eurotium Furocoumarins medicine Food science Chromatography High Pressure Liquid Angelica Principal Component Analysis ABTS biology Chemistry Angelica dahurica 010401 analytical chemistry Reproducibility of Results food and beverages Free Radical Scavengers 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences Solid-state fermentation Polyketides Fermentation Time-of-flight mass spectrometry Food Science |
| Zdroj: | Food Chemistry. 272:670-678 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2018.07.173 |
| Popis: | Eurotium cristatum, a beneficial fungus isolated from Fuzhuan tea, was used to ferment Angelica dahurica for the first time. The antioxidant capacities of the extracts before and after fermentation were compared using ABTS, DPPH and FRAP assays. The results showed that the antioxidant capacities of the extracts acquired using organic solvents were greater after fermentation. Moreover, based on a comparison of the HPLC chromatograms, the chemical composition of Angelica dahurica changed substantially during fermentation. To further understand the changes in its antioxidant constituents, an on-line HPLC-PDA-Triple-TOF-MS/MS-ABTS system was employed. Twelve antioxidants belonging to three different classes were detected and identified, and their antioxidant capacities were preliminarily evaluated. The results indicated that the substances produced during the fermentation of Eurotium cristatum played important roles in enhancing the antioxidant capacity. |
| Databáze: | OpenAIRE |
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