High throughput assay for the determination of lumefantrine in plasma
| Autor: | Anna Annerberg, P. Tipmanee, Niklas Lindegardh, Nicholas J. White, T Singtoroj, N.P.J. Day |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Fluorenes Bioanalysis Lumefantrine Chromatography Clinical Biochemistry Therapeutic drug monitoring studies High Throughput Assay Reproducibility of Results Cell Biology General Medicine Biochemistry Biological fluid Analytical Chemistry Antimalarials chemistry.chemical_compound chemistry Ethanolamines Humans Solid phase extraction Quantitative analysis (chemistry) Chromatography Liquid |
| Popis: | A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1% at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling. |
| Databáze: | OpenAIRE |
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