Screening methylation of DNA repair genes in the oral mucosa of chronic smokers
| Autor: | Patrícia Pimentel de Barros, Janete Dias Almeida, Mônica Ghislaine Oliveira Alves, Antonio Olavo Cardoso Jorge, Fábio Daumas Nunes, Celina Faig Lima Carta, Marcia Sampaio Campos, Jaqueline Scholz |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), University Braz Cubas, Universidade de Mogi das Cruzes, University of Michigan School of Dentistry, Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Epigenomics Male DNA Repair DNA repair Carcinogenesis medicine.disease_cause Real-Time Polymerase Chain Reaction Methylation Andrology 03 medical and health sciences XRCC1 0302 clinical medicine Gene expression PMS2 Medicine Humans Epigenetics General Dentistry Gene Mismatch Repair Endonuclease PMS2 MRE11 Homologue Protein Smokers Repair gene business.industry Smoking Mouth Mucosa Cell Biology General Medicine DNA Methylation 030104 developmental biology MutL Proteins X-ray Repair Cross Complementing Protein 1 Otorhinolaryngology 030220 oncology & carcinogenesis Case-Control Studies Mouth Neoplasms Oral mucosa business |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:37:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01 Objective: The aim of this study was to evaluate the epigenetic changes in the process of oral carcinogenesis by screening the methylation of repair genes in chronic smokers. Design: Two groups were formed: Group 1: 16 smokers with consumption of 20 cigarettes/day for at least 10 years; and Group 2: 10 non-smoking. Exfoliative cytology of the tongue was performed, and the extracted DNA was treated by enzymes. The PCR Array System performed methylation screening to evaluate 22 DNA repair genes, and the results were validated by RT-qPCR for each gene with methylation levels ≥10%. Results: Highest percentages of methylation were observed for MLH3 and XRCC1 genes (11–20% methylation) and in one case for MRE11A and PMS2 (>50% methylation). Statistical analysis showed significant differences in the expression of the genes MRE11A (p = 0.0002), PMS2(p = 0.0068), XRCC1 (p = 0.0080) and MLH3 (0.0057) between the two groups. Conclusion: The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed ≥50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes. Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology University Braz Cubas, Mogi das Cruzes Universidade de Mogi das Cruzes, Mogi das Cruzes Angiogenesis Research Laboratory Department of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry Heart Institute University Hospital Medical School Universidade de São Paulo Department of Oral Pathology School of Dentistry University of Sao Paulo Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology |
| Databáze: | OpenAIRE |
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