Isocitrate metabolism in normal and glucose-6-phosphate dehydrogenase deficient red cells
| Autor: | S.M. King, Yeu-Ming Wang, Jan van Eys |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Electrophoresis
Male Erythrocytes Time Factors Electrophoresis Starch Gel Cell Isocitric acid Glucosephosphate Dehydrogenase Biochemistry chemistry.chemical_compound medicine Animals Humans Glucose-6-phosphate dehydrogenase Cellulose chemistry.chemical_classification Manganese Red Cell Glutathione Hydrogen-Ion Concentration Blood Protein Electrophoresis Chromatography Ion Exchange Creatine Molecular biology Isocitrate Dehydrogenase Kinetics Red blood cell Glucosephosphate Dehydrogenase Deficiency Isocitrate dehydrogenase medicine.anatomical_structure Enzyme chemistry Chromatography Gel Rabbits |
| Zdroj: | Biochemical Medicine. 11:327-337 |
| ISSN: | 0006-2944 |
| Popis: | The G6PD deficient red blood cell does have a normal level of NADP-dependent isocitrate dehydrogenase. The high affinity of this enzyme for isocitrate indicates that even low red cell isocitrate levels could be effective in regenerating NADPH. The enzyme is very stable and is not cell age dependent. The incubation of isocitric acid with the deficient cell resulted in greater GSSG reduction than when glucose was substrate. The injection of isocitric acid and sodium isocitrate was well tolerated and did stimulate significant increases of GSH in rabbits. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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