Mood-Enhancing Antidepressant St. John's Wort Inhibits the Activation of Human Immunodeficiency Virus Gene Expression by Ultraviolet Light
| Autor: | Chad M. Hershey, Mohiuddin M. Taher, Kristoffer Valerie, Guido Lammering |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Gene Expression Regulation
Viral Time Factors MAP Kinase Signaling System Ultraviolet Rays p38 mitogen-activated protein kinases Clinical Biochemistry Biology Transfection p38 Mitogen-Activated Protein Kinases Biochemistry HeLa chemistry.chemical_compound Genes Reporter Gene expression Genetics Ultraviolet light Humans Perylene Molecular Biology Anthracenes Dose-Response Relationship Drug Activator (genetics) NF-kappa B Ginkgo biloba HIV Cell Biology biology.organism_classification Molecular biology Antidepressive Agents humanities Hypericin Butyrates Chloramphenicol chemistry Mitogen-Activated Protein Kinases Signal transduction Hypericum HeLa Cells |
| Zdroj: | IUBMB Life (International Union of Biochemistry and Molecular Biology: Life). 54:357-364 |
| ISSN: | 1521-6551 1521-6543 |
| DOI: | 10.1080/15216540216039 |
| Popis: | Ultraviolet (UV) radiation is a potent activator of the human immunodeficiency virus (HIV) gene expression in a HeLa cell clone with stably integrated copies of the HIVcat reporter construct. Recently, we have shown that activation of p38 MAP kinase and NF-kappaB is necessary but not sufficient for triggering efficient HIV gene expression in response to UV. Here we demonstrate that St. John's wort is a potent inhibitor of the UV-induced activation of HIV gene expression in HeLa cells. Stably transfected HIVcat/HeLa cells were preincubated with different amounts (25-100 microl) of St. John's wort or gingko biloba extracts for 30 min, then irradiated with UV (30 J/m2). In contrast to ginkgo biloba, St. John's wort inhibited the UV-induced HIV gene expression in a dose-dependent manner. Furthermore, preincubation with St. John's wort (10, 20, and 30 microl) for 30 min before UV (30 J/m2) irradiation, PMA- and UV-induced NF-kappaB activation was completely blocked, whereas ginkgo biloba did not affect the PMA- and UV-induced NF-kappaB activation in HeLa cells. UV activation of p38 MAP kinase was not inhibited by St. John's wort or by ginkgo biloba. However, we found that p38 MAP kinase and JNK1 and -2 were activated by St. John's wort, but p44/42 MAP kinase was not activated by St. John's wort in HeLa cells. Hypericin an active ingredient in St. John's wort also inhibited the UV activation of HIV gene expression in HeLa cells. These results firmly confirm that St. John's wort is a potent inhibitor of the UV-induced activation of HIV gene expression in HeLa cells. |
| Databáze: | OpenAIRE |
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