Epidermal growth factor receptor-targeted lipid nanoparticles retain self-assembled nanostructures and provide high specificity
| Autor: | Judith A. Scoble, Benjamin W. Muir, Nhiem Tran, Nan Li, Gregory Coia, Calum J. Drummond, Lynne J. Waddington, George O. Lovrecz, Jiali Zhai, Nigel Kirby, Xavier Mulet |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
Nanoparticle Nanotechnology Conjugated system Ligands Maleimides Immunoglobulin Fab Fragments Amphiphile Humans Scattering Radiation General Materials Science Sulfhydryl Compounds Epidermal growth factor receptor Particle Size Drug Carriers Liposome biology Phosphatidylethanolamines X-Rays Ligand binding assay Cryoelectron Microscopy Lipids Recombinant Proteins Liquid Crystals ErbB Receptors Liposomes Drug delivery biology.protein Biophysics Nanoparticles Fatty Alcohols Drug carrier |
| Zdroj: | Nanoscale. 7:2905-2913 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c4nr05200e |
| Popis: | Next generation drug delivery utilising nanoparticles incorporates active targeting to specific sites. In this work, we combined targeting with the inherent advantages of self-assembled lipid nanoparticles containing internal nano-structures. Epidermal growth factor receptor (EGFR)-targeting, PEGylated lipid nanoparticles using phytantriol and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG-maleimide amphiphiles were created. The self-assembled lipid nanoparticles presented here have internal lyotropic liquid crystalline nano-structures, verified by synchrotron small angle X-ray scattering and cryo-transmission electron microscopy, that offer the potential of high drug loading and enhanced cell penetration. Anti-EGFR Fab' fragments were conjugated to the surface of nanoparticles via a maleimide-thiol reaction at a high conjugation efficiency and retained specificity following conjugation to the nanoparticles. The conjugated nanoparticles were demonstrated to have high affinity for an EGFR target in a ligand binding assay. |
| Databáze: | OpenAIRE |
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