Different Growth Properties of Neointimal and Medial Smooth Muscle Cells in Response to Growth Factors

Autor: Patrick Delafontaine, Samir Myit, Stéphane Giraud, Marijke Brink, Giulio Gabbiani, Marie-Luce Bochaton-Piallat
Rok vydání: 2003
Předmět:
Platelet-derived growth factor
Proto-Oncogene Proteins c-jun
Physiology
medicine.medical_treatment
Basic fibroblast growth factor
Fibroblast Growth Factor 2/pharmacology
Aorta
Thoracic
Proto-Oncogene Proteins c-fos/genetics
ddc:616.07
Fibroblast growth factor
Insulin-Like Growth Factor I/genetics/pharmacology
Muscle
Smooth
Vascular
chemistry.chemical_compound
Growth Substances/ pharmacology
Tunica Intima/cytology/drug effects
Insulin-Like Growth Factor I
Growth Substances
Cells
Cultured
Insulin-Like Growth Factor Binding Proteins/genetics
Platelet-Derived Growth Factor
RNA
Messenger/analysis
Receptors
Growth Factor/genetics
Cell biology
Insulin-Like Growth Factor Binding Proteins
Fibroblast Growth Factor 2
Cardiology and Cardiovascular Medicine
Proto-Oncogene Proteins c-fos
Epithelioid cell
Cell Division
Platelet-Derived Growth Factor/pharmacology
Neointima
medicine.medical_specialty
Cell type
Muscle
Smooth
Vascular/ cytology/ drug effects
Cell Division/drug effects
Biology
Genes
Immediate-Early/physiology
Internal medicine
medicine
Animals
Receptors
Growth Factor
RNA
Messenger
Genes
Immediate-Early
Growth factor
Aorta
Thoracic/cytology
Proto-Oncogene Proteins c-jun/genetics
Rats
Endocrinology
chemistry
Cell culture
Tunica Intima
Zdroj: Journal of Vascular Research, Vol. 40, No 2 (2003) pp. 97-104
ISSN: 1423-0135
1018-1172
DOI: 10.1159/000070706
Popis: Different smooth muscle cell phenotypes coexist in arteries. The majority of cells cultured from a normal rat aortic media have a spindle-shaped phenotype while cells isolated from an intimal thickening 15 days after endothelial injury show a distinct epithelioid phenotype. These two phenotypes express their own specific set of genes and differ in their proliferation and migration characteristics. We studied growth factor-induced DNA synthesis in both phenotypes and investigated the potential mechanisms behind the differences in growth characteristics. Insulin-like growth factor-I (IGF-I), platelet-derived growth factor (PDGF-BB), and basic fibroblast growth factor (FGF) increased thymidine incorporation in both phenotypes, but the increase was markedly stronger in neointimal cells than in medial cells. Northern blot analysis 30 min after growth factor stimulation showed that c-fos and c-jun mRNAs were induced more strongly in neointimal than in medial cells. IGF-I receptor and PDGF-Rβ levels were higher in neointimal cells than in medial cells, but the FGF receptor level was not different between the cell types. In summary, our results indicate that neointimal cells are more sensitive to growth factors than medial cells, likely due to a higher expression of IGF-I receptor and PDGF-Rβ. Our results provide insight into the mechanism by which epithelioid cells play a primary role in vascular neointima formation.
Databáze: OpenAIRE
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