Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity
| Autor: | Hongxia Niu, Bingdong Zhu, He Juanjuan, Junxia Tang, Jiangyuan Han, Lijun Zhou, Chunxiang Bai |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Tuberculosis Immunology lcsh:Medicine Article T cell mediated immunity central memory like T cells Mycobacterium tuberculosis 03 medical and health sciences 0302 clinical medicine Immune system Drug Discovery fusion cytokines medicine M. tuberculosis Pharmacology (medical) Pharmacology Mycobacterium bovis biology business.industry lcsh:R biology.organism_classification medicine.disease IL-7-Linker-IL-15 Vaccination 030104 developmental biology Infectious Diseases Immunization Interleukin 15 subunit vaccine business 030215 immunology |
| Zdroj: | Vaccines, Vol 8, Iss 715, p 715 (2020) Vaccines Volume 8 Issue 4 |
| Popis: | Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), is among the most serious infectious diseases worldwide. Adjuvanted protein subunit vaccines have been demonstrated as a kind of promising novel vaccine. This study proposed to investigate whether cytokines interliukine-7 (IL-7) and interliukine-15 (IL-15) help TB subunit vaccines induce long-term cell-mediated immune responses, which are required for vaccination against TB. In this study, mice were immunized with the M. tuberculosis protein subunit vaccines combined with adnovirus-mediated cytokines IL-7, IL-15, IL-7-IL-15, and IL-7-Linker-IL-15 at 0, 2, and 4 weeks, respectively. Twenty weeks after the last immunization, the long-term immune responses, especially the central memory-like T cells (TCM like cell)-mediated immune responses, were determined with the methods of cultured IFN-&gamma ELISPOT, expanded secondary immune responses, cell proliferation, and protective efficacy against Mycobacterium bovis Bacilli Calmette-Guerin (BCG) challenge, etc. The results showed that the group of vaccine + rAd-IL-7-Linker-IL-15 induced a stronger long-term antigen-specific TCM like cells-mediated immune responses and had higher protective efficacy against BCG challenge than the vaccine + rAd-vector control group, the vaccine + rAd-IL-7 and the vaccine + rAd-IL-15 groups. This study indicated that rAd-IL-7-Linker-IL-15 improved the TB subunit vaccine&rsquo s efficacy by augmenting TCM like cells and provided long-term protective efficacy against Mycobacteria. |
| Databáze: | OpenAIRE |
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