Late changes in blood-brain barrier permeability in a rat tMCAO model of stroke detected by gadolinium-enhanced MRI
| Autor: | Diana Cash, John S Beech, Elaine A. Irving, Michel Mesquita, Steve C.R. Williams, Maria Ashioti, Catherine Morgan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Time Factors Gadolinium Ischemia chemistry.chemical_element Contrast Media Blood–brain barrier Capillary Permeability Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals cardiovascular diseases Stroke business.industry Infarction Middle Cerebral Artery General Medicine medicine.disease Image Enhancement Magnetic Resonance Imaging Disease Models Animal 030104 developmental biology medicine.anatomical_structure nervous system Neurology chemistry Blood-Brain Barrier cardiovascular system Cardiology Cerebral ischaemia Neurology (clinical) Blood brain barrier permeability business 030217 neurology & neurosurgery |
| Zdroj: | Neurological research. 42(10) |
| ISSN: | 1743-1328 |
| Popis: | After cerebral ischaemia the blood-brain barrier (BBB) may be compromised and this has been observed in both clinical and preclinical studies. The timing of BBB disruption after ischaemia has long been considered to be biphasic, however some groups contest this view. Therefore, the purpose of this study was to characterize the BBB permeability timecourse in a rat model at both acute and chronic time points.Unilateral transient middle cerebral artery occlusion (tMCAO) was performed in 15 male Sprague Dawley rats. Change in T1-weighted MR signal before and after an injection of gadolinium-based contrast agent was calculated voxelwise to derive a BBB permeability index (BBBPI) at both early (6 h, 12 h, and 24 h) and late (7 and 14 days) time points.As expected, BBBPI in the non-lesioned ROI was not significantly different from pre-occlusion baseline at any time point. However, BBBPI in the ipsilateral (lesioned) ROI was statistically different to baseline at day 7 (p 0.001) and day 14 (p 0.01) post-tMCAO. There was a small, but not-significant increase in BBBPI in the earlier phase (at 6 hours).Our results indicate a significant late opening of the BBB. This is important as the majority of previous studies have only characterised an early acute BBB permeability in ischemia. However, the later period of increased permeability may indicate an optimal time for drug delivery across the BBB, when it is especially suited to drugs targeting delayed processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|