Simultaneous analysis of saturated and unsaturated fatty acids present in pequi fruits by capillary electrophoresis
| Autor: | Ana Vládia Bandeira Moreira, Cristiane do Carmo Cesário, Leandro de Morais Cardoso, Patrícia Mendonça de Castro Barra, Helena Maria Pinheiro-Sant'Ana, Maria do Carmo Gouveia Peluzio, Marcone Augusto Leal de Oliveira, Bárbara Nery-Enes |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Response factor
Gas chromatography Chromatography Capillary zone electrophoresis pequi fruit Pulp (paper) gas chromatography Extraction (chemistry) General Chemistry Electrolyte engineering.material lcsh:Chemistry chemistry.chemical_compound Capillary electrophoresis chemistry lcsh:QD1-999 capillary zone electrophoresis engineering Pequi fruit Derivatization Acetonitrile |
| Zdroj: | Química Nova, Vol 36, Iss 9, Pp 1430-1433 (2013) LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1678-7064 |
| Popis: | O artigo não contém o resumo em português. In the current study, an alternative method has been proposed for simultaneous analysis of palmitic, stearic, oleic, linoleic, and linolenic acids by capillary zone electrophoresis (CZE) using indirect detection. The background electrolyte (BGE) used for the analysis of these fatty acids (FAs) consisted of 15.0 mmol L^−1 NaH2PO4/Na2HPO4 at pH 6.86, 4.0 mmol L^−1 SDBS, 8.3 mmol L^−1 Brij 35, 45% v/v acetonitrile (can), and 2.1% n-octanol. The FAs quantification of FAs was performed using a response factor approach, which provided a high analytical throughput for the real sample. The CZE method, which was applied successfully for the analysis of pequi pulp, has advantages such as short analysis time, absence of lipid fraction extraction and derivatization steps, and no significant difference in the 95% confidence intervals for FA quantification results, compared to the gas chromatography official method (AOCS Ce 1h-05). |
| Databáze: | OpenAIRE |
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