Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability
| Autor: | Anton Horváth, Pavel Flegontov, Roman Sobotka, Francisco J. Ayala, Anna Gnipová, Julius Lukeš, Luděk Kořený, Miroslav Oborník, Julie Kovářová |
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| Rok vydání: | 2012 |
| Předmět: |
Porphyrins
food.ingredient Hemeprotein Phytomonas Respiratory chain Crithidia fasciculata Heme Biology Models Biological Cofactor Electron Transport Lanosterol chemistry.chemical_compound food Ergosterol Kinetoplastida Phylogeny Multidisciplinary Fatty Acids Metabolism Biological Sciences Oxygen Oxidative Stress Sterols Metabolic pathway Biochemistry chemistry biology.protein Trypanosomatina Oxidation-Reduction |
| Zdroj: | Kořený, L; Sobotka, R; Kovářová, J; Gnipová, A; Flegontov, P; Horváth, A; et al.(2012). Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability. Proceedings of the National Academy of Sciences of the United States of America, 109(10), 3808-3813. doi: 10.1073/pnas.1201089109. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/4d39w5gd |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Heme is an iron-coordinated porphyrin that is universally essential as a protein cofactor for fundamental cellular processes, such as electron transport in the respiratory chain, oxidative stress response, or redox reactions in various metabolic pathways. Parasitic kinetoplastid flagellates represent a rare example of organisms that depend on oxidative metabolism but are heme auxotrophs. Here, we show that heme is fully dispensable for the survival of Phytomonas serpens , a plant parasite. Seeking to understand the metabolism of this heme-free eukaryote, we searched for heme-containing proteins in its de novo sequenced genome and examined several cellular processes for which heme has so far been considered indispensable. We found that P. serpens lacks most of the known hemoproteins and does not require heme for electron transport in the respiratory chain, protection against oxidative stress, or desaturation of fatty acids. Although heme is still required for the synthesis of ergosterol, its precursor, lanosterol, is instead incorporated into the membranes of P. serpens grown in the absence of heme. In conclusion, P. serpens is a flagellate with unique metabolic adaptations that allow it to bypass all requirements for heme. |
| Databáze: | OpenAIRE |
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