Compartment-Dependent Colocalization of Kir3.2-Containing K+Channels and GABABReceptors in Hippocampal Pyramidal Cells
| Autor: | Cheryl L. Marker, Yu Kasugai, Kevin Wickman, Michael Frotscher, Bernhard Bettler, Akos Kulik, Franck Rigato, Imre Vida, Nicole Guetg, Yugo Fukazawa, Ryuichi Shigemoto |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Mice Knockout Dendritic spine Pyramidal Cells General Neuroscience Colocalization Articles GABAB receptor Biology Hippocampal formation Hippocampus Rats Cell biology Mice medicine.anatomical_structure Metabotropic receptor G Protein-Coupled Inwardly-Rectifying Potassium Channels Receptors GABA-B Excitatory postsynaptic potential medicine Animals Rats Wistar Pyramidal cell G protein-coupled receptor |
| Zdroj: | The Journal of Neuroscience. 26:4289-4297 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.4178-05.2006 |
| Popis: | G-protein-coupled inwardly rectifying K+channels (Kir3 channels) coupled to metabotropic GABABreceptors are essential for the control of neuronal excitation. To determine the distribution of Kir3 channels and their spatial relationship to GABABreceptors on hippocampal pyramidal cells, we used a high-resolution immunocytochemical approach. Immunoreactivity for the Kir3.2 subunit was most abundant postsynaptically and localized to the extrasynaptic plasma membrane of dendritic shafts and spines of principal cells. Quantitative analysis of immunogold particles for Kir3.2 revealed an enrichment of the protein around putative glutamatergic synapses on dendritic spines, similar to that of GABAB1. Consistent with this observation, a high degree of coclustering of Kir3.2 and GABAB1was revealed around excitatory synapses by the highly sensitive SDS-digested freeze–fracture replica immunolabeling. In contrast, in dendritic shafts receptors and channels were found to be mainly segregated. These results suggest that Kir3.2-containing K+channels on dendritic spines preferentially mediate the effect of GABA, whereas channels on dendritic shafts are likely to be activated by other neurotransmitters as well. Thus, Kir3 channels, localized to different subcellular compartments of hippocampal principal cells, appear to be differentially involved in synaptic integration in pyramidal cell dendrites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|