Rapid Exchange of A:T Base Pairs Is Essential for Recognition of DNA Homology by Human Rad51 Recombination Protein
| Autor: | Masayuki Takahashi, Ewa Folta-Stogniew, Charles M. Radding, Shawn M. O'Malley, Ravindra C. Gupta |
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| Rok vydání: | 1999 |
| Předmět: |
Guanine
DNA Repair Base pair DNA repair Base Pair Mismatch RAD51 Biology Homology (biology) Fluorescence chemistry.chemical_compound Cytosine D-loop Sequence Homology Nucleic Acid Humans Crossing Over Genetic Base Pairing Molecular Biology Genetics Base Sequence Adenine DNA Cell Biology Inosine DNA-Binding Proteins Kinetics Förster resonance energy transfer chemistry Oligodeoxyribonucleotides Mutation Rad51 Recombinase Recombination Thymine |
| Zdroj: | Molecular Cell. 4(5):705-714 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/s1097-2765(00)80381-0 |
| Popis: | Human Rad51 belongs to a ubiquitous family of proteins that enable a single strand to recognize homology in duplex DNA, and thereby to initiate genetic exchanges and DNA repair, but the mechanism of recognition remains unknown. Kinetic analysis by fluorescence resonance energy transfer combined with the study of base substitutions and base mismatches reveals that recognition of homology, helix destabilization, exchange of base pairs, and initiation of strand exchange are integral parts of a rapid, concerted mechanism in which A:T base pairs play a critical role. Exchange of base pairs is essential for recognition of homology, and physical evidence indicates that such an exchange occurs early enough to mediate recognition. |
| Databáze: | OpenAIRE |
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