miR‐451a is downregulated and targets PSMB8 in prostate cancer
| Autor: | Huan‐Zhi Yang, Yong‐Jun Jiang, Li‐Qi Xu, Yun Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Proteasome Endopeptidase Complex Apoptosis PSMB8 colony formation 03 medical and health sciences 0302 clinical medicine Western blot Antigens CD Cell Movement Genes Reporter Cell Line Tumor microRNA Humans Medicine Luciferase Luciferases Base Pairing lcsh:R5-920 Reporter gene Oligoribonucleotides miR‐451a Base Sequence medicine.diagnostic_test business.industry Cell growth Molecular Mimicry Prostate General Medicine Cadherins cell invasion prostate cancer Gene Expression Regulation Neoplastic MicroRNAs cell proliferation Cell culture 030220 oncology & carcinogenesis Cancer research 030211 gastroenterology & hepatology lcsh:Medicine (General) business Plasmids Signal Transduction |
| Zdroj: | Kaohsiung Journal of Medical Sciences, Vol 36, Iss 7, Pp 494-500 (2020) |
| ISSN: | 2410-8650 1607-551X |
| DOI: | 10.1002/kjm2.12196 |
| Popis: | Abnormal expression of microRNAs (miRNAs) is frequently occurred in prostate cancer (PCa). This study was aimed to investigate the biological roles of miR‐451a in PCa. Quantitative real‐time PCR (qRT‐PCR) and Western blot were employed to investigate the expression levels of miR‐451a and proteasome (prosome, macropain) subunit, beta type, 8 (PSMB8) in PCa cell lines. Luciferase activity reporter assay was used to verify the connection between miR‐451a and PSMB8. in vitro functional experiments were performed to measure the effects of miR‐451a or PSMB8 on PCa cell proliferation, colony formation ability, cell invasion, and cell apoptosis. miR‐451a expression was downregulated, whereas PSMB8 expression was upregulated in PCa cell lines. Luciferase activity reporter assay confirmed the direct connection between miR‐451a and PSMB8. Overexpression of miR‐451a inhibits PCa cell proliferation, colony formation, cell invasion and promotes cell apoptosis, while the overexpression of PSMB8 caused the opposite effects. Moreover, rescue experiments confirmed PSMB8 was a functional target of miR‐451a. In conclusion, this study provides novel insights into the role of miR‐451a in PCa, and the results demonstrated miR‐451a could inhibit PCa progression by targeting PSMB8. |
| Databáze: | OpenAIRE |
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