Intra- and interlaboratory performance of antibiotic disk-diffusion-susceptibility testing of bacterial control strains of relevance for monitoring aquaculture environments
| Autor: | Geert Huys, Nguyen Thanh Phuong, Jean Swings, Mohamed Shariff, Kerry L. Bartie, Mauro Giacomini, Supranee Chinabut, Margo Cnockaert, S. Bertone, Alan J. Teale, Fatimah Md Yussoff, Dang Thi Hoang Oanh, Temdoung Somsiri |
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| Rok vydání: | 2005 |
| Předmět: |
International level
Susceptibility testing Bacteria Intralaboratory business.industry Aquaculture Microbial Sensitivity Tests Reference Standards Aquatic Science Biology Anti-Bacterial Agents Culture Media Central laboratory Diffusion Evaluation Studies as Topic Drug Resistance Bacterial Statistics media_common.cataloged_instance European union business Ecology Evolution Behavior and Systematics Standard operating procedure media_common Arithmetic mean |
| Zdroj: | Diseases of Aquatic Organisms. 66:197-204 |
| ISSN: | 1616-1580 0177-5103 |
| Popis: | In the course of an international research project on hazard analysis of antimicrobial resis- tance in SE Asian aquaculture environments, 2 European Union and 3 SE Asian laboratories attempted to harmonize a procedure for antimicrobial agent susceptibility testing based on disk diffusion (DD). For this purpose, a selected panel of 10 bacterial control strains of relevance for monitoring warm-water aquacul- ture environments was sent by the central laboratory to the other participating laboratories. In each lab- oratory, 10 independently replicated DD determinations of each control strain to 6 antibiotics were per- formed using Iso-Sensitest Agar (ISA) according to a standard operating procedure (SOP); in total, this study thus yielded 300 data sets for all 5 laboratories. At the end of the study, strain authenticity of sub- cultures of the control strains used by the respective participating laboratories was verified by the central laboratory. Based on the arithmetic mean of 10 inhibition-zone diameter measurements and standard deviation (SD), intralaboratory SD variations ranged from 0 to 2 mm when 79% of the recorded data sets were considered. In 8% of the data sets, the SD value exceeded 4 mm, which in most cases could be attributed to the fact that the data points for a given strain-disk combination were not normally distrib- uted in one of the laboratories. At the interlaboratory level, 81% of the SD values based on global aver- aging of 50 data points per strain-disk combination were situated in the 0 to 5 mm range. Comparison with a minimal data set from literature of DD testing performed with Mueller-Hinton (MH) medium indi- cated that the use of either ISA or MH medium in DD testing has a limited impact on the method's preci- sion among different laboratories. In conclusion, the current study has provided a validated SOP to pro- mote the coordination and harmonization of DD-susceptibility methodologies for aquaculture-associated organisms at an international level. As one of the main action items for the future, new interpretive break- points should be specifically designed and validated for aquaculture drugs and organisms. |
| Databáze: | OpenAIRE |
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