Comparison of characteristics of long noncoding RNA in Hanwoo according to sex
| Autor: | Seungwoo Son, Donghyun Shin, Jae-Young Choi, Jae-Don Oh, Kyeong Hye Won |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
muscle
lcsh:Animal biochemistry Quantitative trait locus Biology Genome Article 03 medical and health sciences Gene expression sex lcsh:QP501-801 Gene lcsh:SF1-1100 030304 developmental biology Genetics 0303 health sciences 0402 animal and dairy science RNA 04 agricultural and veterinary sciences Animal Breeding and Genetics 040201 dairy & animal science Long non-coding RNA hanwoo lncrna quantitative trait loci (qtl) Hanwoo Animal Science and Zoology lcsh:Animal culture Function (biology) Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 33, Iss 5, Pp 696-703 (2020) Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| DOI: | 10.5713/ajas.18.0533 |
| Popis: | Objective: Cattle were some of the first animals domesticated by humans for the production of milk, meat, etc. Long noncoding RNA (lncRNA) is defined as longer than 200 bp in non-protein coding transcripts. lncRNA is known to function in regulating gene expression and is currently being studied in a variety of livestock including cattle. The purpose of this study is to analyze the characteristics of lncRNA according to sex in Hanwoo cattle.Methods: This study was conducted using the skeletal muscles of 9 Hanwoo cattle include bulls, steers and cows. RNA was extracted from skeletal muscle of Hanwoo. Sequencing was conducted using Illumina HiSeq2000 and mapped to the Bovine Taurus genome. The expression levels of lncRNAs were measured by DEGseq and quantitative trait loci (QTL) data base was used to identify QTLs associated with lncRNA. The python script was used to match the nearby genesResults: In this study, the expression patterns of transcripts of bulls, steers and cows were identified. And we identified significantly differentially expressed lncRNAs in bulls, steers and cows. In addition, characteristics of lncRNA which express differentially in muscles according to the sex of Hanwoo were identified. As a result, we found differentially expressed lncRNAs according to sex were related to shear force and body weight.Conclusion: This study was classified and characterized lncRNA which differentially expressed by sex in Hanwoo cattle. We believe that the characterization of lncRNA by sex of Hanwoo will be helpful for future studies of the physiological mechanisms of Hanwoo cattle. |
| Databáze: | OpenAIRE |
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