Comparison of 22C3 PD-L1 Expression between Surgically Resected Specimens and Paired Tissue Microarrays in Non–Small Cell Lung Cancer
| Autor: | Kan Jiang, Qian Miao, Haipeng Xu, Weifeng Zhu, Cheng Huang, Peisha Huang, Wu Zhuang, Xiaohui Chen, Dan Hu, Chao Li, Gen Lin, Tony Mok, Yun-jian Huang, Xiandong Lin, Xirong Fan |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Lung Neoplasms Pembrolizumab B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Paired samples Carcinoma Non-Small-Cell Lung Biopsy Biomarkers Tumor medicine Humans Lung cancer Aged Tissue microarray medicine.diagnostic_test business.industry Middle Aged medicine.disease 030104 developmental biology Oncology Tissue Array Analysis 030220 oncology & carcinogenesis Immunohistochemistry Pd l1 expression Non small cell business Nuclear medicine |
| Zdroj: | Journal of Thoracic Oncology. 12:1536-1543 |
| ISSN: | 1556-0864 |
| DOI: | 10.1016/j.jtho.2017.07.015 |
| Popis: | The extent to which intratumoral heterogeneity of programmed death ligand 1 (PD-L1) expression causes discordance of PD-L1 expression between paired samples remains unclear. Here, PD-L1 status was compared between whole sections from NSCLCs and the corresponding tissue microarrays (TMAs) serving as surrogate biopsy specimens.PD-L1 expression was evaluated by 22C3 immunohistochemistry assay on 190 archival surgical specimens and matched to the TMA results. PD-L1 expression was determined by the tumor proportion score (TPS) and classified as TPS lower than 1%, TPS of 1% to 49%, and TPS of 50% or higher. Agreement statistics were used.The percentage of PD-L1 expression on tumor cells differed greatly between individual TMAs and matched surgical specimens. When PD-L1 TPS was adopted, a total of 36 of 190 discordance cases (18.9%) were observed, with a κ-value of 0.630 between paired samples. The TMAs underestimated or overestimated PD-L1 status in 19 of 36 (52.8%) and 17 of 36 (47.2%) of the matched surgical specimens, respectively (p = 0.118). The discordance rate was much lower in cases with a PD-L1 TPS lower than 1% compared with in cases with a TPS of 1% to 49% and TPS of 50% or higher (18.4% versus 56.7% and 43.3%, p0.001). When a TPS of 50% or higher was used as the cutoff, the discordance rate of PD-L1 TPS less than 50% was further reduced to 7.5%. Such discrepancies were due mainly to intratumoral heterogeneity of PD-L1 expression and nonsignificant association with clinicopathological features.PD-L1 expression in TMAs correlates moderately well with that in the corresponding surgical specimens, indicating that evaluating PD-L1 expression in diagnostic biopsy specimens could be misleading in defining sensitivity to pembrolizumab treatment yet may be reliable as a way to exclude patients with a PD-L1 TPS less than 50% from first-line pembrolizumab treatment. |
| Databáze: | OpenAIRE |
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