Pectin nanoparticle enhances cytotoxicity of methotrexate against hepG2 cells
Autor: | Montree Jaturanpinyo, Supachoke Mangmool, Chuda Chittasupho |
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Rok vydání: | 2012 |
Předmět: |
Antimetabolites
Antineoplastic animal structures food.ingredient Materials science Pectin Pharmaceutical Science Nanoparticle Conjugated system complex mixtures chemistry.chemical_compound Drug Delivery Systems food medicine Humans Cytotoxicity Carbodiimide Cytotoxins digestive oral and skin physiology food and beverages Hep G2 Cells General Medicine Controlled release Methotrexate Biochemistry chemistry Drug delivery Biophysics Nanoparticles Pectins medicine.drug |
Zdroj: | Drug Delivery. 20:1-9 |
ISSN: | 1521-0464 1071-7544 |
Popis: | This work has aimed to develop methotrexate-conjugated pectin nanoparticle for delivering a cytotoxic drug to hepatic cancer cell.Methotrexate was conjugated to pectin by carbodiimide chemistry. Nanoparticles of pectin conjugated with methotrexate (MTX) were then fabricated by using ionotropic gelation. The size, shape and surface charge were characterized by dynamic light scattering and microscopic method. Cytotoxicity of MTX-pectin nanoparticle was monitored by MTT assay.Methotrexate-pectin nanoparticle was successfully formulated. Drug release study indicated that MTX-NP exhibited sustained drug release at pH 7.4. Sustained release of methotrexate may enable methotrexate-pectin nanoparticle as a controlled drug delivery system. Cytotoxicity study confirmed the activity of the drug incorporated in nanoparticles. In addition, the cytotoxicity of methotrexate was increased when conjugated to pectin nanoparticles, compared to free methotrexate.This study verified that pectin can deliver methotrexate to hepatic cancer cell and provide sustained drug delivery. The cytotoxicity of methotrexate was enhanced when methotrexate was conjugated to pectin indicating the improved drug delivery to cancer cell. |
Databáze: | OpenAIRE |
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