Risk of Chlamydia abortus transmission via embryo transfer using in vitro produced early bovine embryos

Autor: M. Oseikria, Karine Laroucau, J.L. Pellerin, Fabien Vorimore, Cécile Roux, Diego Moreno, Jean-Fancois Bruyas, Sandrine Michaud, Annie Rodolakis, M. Larrat, Francis Fieni
Přispěvatelé: Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Laboratoire de santé animale, Unité de zoonose bactérienne, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), LUNAM Université [Nantes Angers Le Mans], Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
animal structures
040301 veterinary sciences
[SDV]Life Sciences [q-bio]
Cattle Diseases
Chlamydia abortus
Fertilization in Vitro
Risk Assessment
0403 veterinary science
Andrology
IETS
03 medical and health sciences
Food Animals
medicine
Animals
Bovine embryo
Chlamydia
Small Animals
Zona pellucida
Incubation
030304 developmental biology
0303 health sciences
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
biology
Equine
Chemistry
Embryo
Embryo transfer
04 agricultural and veterinary sciences
Chlamydia Infections
biology.organism_classification
Embryo
Mammalian
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
In vitro
3. Good health
medicine.anatomical_structure
PCR
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
embryonic structures
Animal Science and Zoology
Cattle
Bacteria
Zdroj: Theriogenology
Theriogenology, Elsevier, 2019, 126, pp.114-120. ⟨10.1016/j.theriogenology.2018.11.033⟩
Theriogenology, 2019, 126, pp.114-120. ⟨10.1016/j.theriogenology.2018.11.033⟩
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2018.11.033⟩
Popis: International audience; The objectives of this study were to determine (i) whether Chlamydia (C) abortus would adhere to the intact zona pellucida (ZP-intact) of early in vitro produced bovine embryos; (ii) whether the bacteria would adhere to the embryos (ZP-free) after in vitro infection; and (iii) the efficacy of the International Embryo Transfer Society (LETS) washing protocol.The experimentation was made twice. For each replicate 100 (8-16-cell) bovine embryos produced in vitro were randomly divided into 10 batches. Height batches (4 ZP-intact and 4 ZP-free) of 10 embryos were incubated in a medium containing 4 x 10(7) Chlamydia/ml of AB7 strain. After incubation for 18 h at 37 degrees C in an atmosphere of 5% CO2, the embryos were washed in accordance with the LETS guidelines. In parallel, two batches (1 ZP-intact and 1 ZP-free) of 10 embryos were subjected to similar procedures but without exposure to C abortus as a control group. The 10 washing fluids from each batch were collected and centrifuged for 1 h at 13,000 xg. Each batch of washed embryos and each wash pellets were tested using PCR.C abortus DNA was found in all ZP-intact and ZP-free batches of 10 embryos after 10 successive washes. For ZP-intact infected embryos, Chlamydia-DNA was also detected in all 10 wash baths for two batches (2/8) of embryos, whereas for ZP-free infected embryos, Chlamydia-DNA was detected in all 10 wash baths for 6/8 batches of embryos. In contrast, none of the embryos or their washing fluids in the control batches was DNA positive. The bacterial load for batches of 10 embryos after the 10 wash baths was significantly higher for batches of ZP-free embryos (20.7 +/- 9 x 10(3) bacteria/mL) than for batches of ZP-intact embryos (0.47 +/- 0.19 x 10(3) bacteria/mL).These results demonstrate that C. abortus adheres to the ZP as well as the early embryonic cells of in vitro produced bovine embryos after in vitro infection, and that the standard washing protocol recommended by the LETS fails to remove it.
Databáze: OpenAIRE
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