Decreased Bacterial Adherence, Biofilm Formation, and Tissue Reactivity of Barbed Monofilament Suture in an In Vivo Contaminated Wound Model
| Autor: | Christopher Bergum, David C. Markel, Nancy Jackson, Michael R. Morris |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus aureus
medicine.medical_specialty medicine.disease_cause Bacterial Adhesion Protein expression Microbiology 03 medical and health sciences 0302 clinical medicine Suture (anatomy) In vivo medicine Animals Surgical Wound Infection Orthopedics and Sports Medicine Mice Inbred BALB C 030222 orthopedics Sutures Tumor Necrosis Factor-alpha business.industry Suture Techniques Biofilm Histology Staphylococcal Infections Braided suture Surgery Biofilms 030220 oncology & carcinogenesis Monofilament suture Microscopy Electron Scanning Female business Interleukin-1 |
| Zdroj: | The Journal of Arthroplasty. 32:1272-1279 |
| ISSN: | 0883-5403 |
| Popis: | Background Monofilament and barbed monofilament sutures have been shown in in vitro models to have less bacterial adherence than braided suture. This study evaluates bacterial adherence to suture materials and tissue reactivity with an in vivo contaminated wound mouse model. Methods Staphylococcus aureus was used to create an in vivo contaminated wound model at 2 amounts (10 6 colony-forming units [CFU] and 10 8 CFU) using a mouse air pouch. Three types of commonly used absorbable suture were evaluated: braided, monofilament, and barbed monofilament. Bacterial suture adherence was evaluated with suture culture, a photon-capturing camera system, and scanning electron microscopy. Tissue reactivity was assessed through histology and protein expression. Results The braided suture group with the high amount of S aureus exhibited frank purulence and air pouch hypertrophy in all 8 mice. A significant difference was found between suture groups inoculated with 10 8 CFU ( P P P P P Conclusion Our in vivo contaminated wound model demonstrated that barbed monofilament suture performed similarly to monofilament suture and better than braided suture in terms of bacterial adherence, biofilm formation, and tissue reactivity. |
| Databáze: | OpenAIRE |
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