A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity
| Autor: | Natasha Kondratieva, Anneke Funk, Shessy Torres, J. Leung, Roy A. Hall, Gorben P. Pijlman, Ann C. Palmenberg, Wen Jun Liu, Lieke van der Aa, Pei Yong Shi, Alexander A. Khromykh |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Cancer Research MICROBIO RNA Untranslated RNA-induced transcriptional silencing viruses Laboratory of Virology RNA-dependent RNA polymerase Celbiologie en Immunologie Viral Plaque Assay Biology cyclization sequences Microbiology encephalitis-virus Laboratorium voor Virologie untranslated region Mice Ribonucleases Cytopathogenic Effect Viral Virology Immunology and Microbiology(all) genetic algorithm Animals apoptotic pathway 3' Untranslated Regions Molecular Biology Genetics kunjin virus EPS-2 Flavivirus Intron RNA secondary structure unfolded protein response Non-coding RNA Noncoding DNA RNA silencing Cell Biology and Immunology RNA editing west-nile-virus Nucleic Acid Conformation RNA Viral saccharomyces-cerevisiae Parasitology |
| Zdroj: | Cell Host & Microbe 4 (2008) 6 Cell Host & Microbe, 4(6), 579-591 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2008.10.007 |
| Popis: | SummaryViral noncoding RNAs have been shown to play an important role in virus-host interplay to facilitate virus replication. We report that members of the genus Flavivirus, a large group of medically important encephalitic RNA viruses, produce a unique and highly structured noncoding RNA of 0.3–0.5 kb derived from the 3′ untranslated region of the viral genome. Using West Nile virus as a model, we show that this subgenomic RNA is a product of incomplete degradation of viral genomic RNA by cellular ribonucleases. Highly conserved RNA structures located at the beginning of the 3′ untranslated region render this RNA resistant to nucleases, and the resulting subgenomic RNA product is essential for virus-induced cytopathicity and pathogenicity. Thus, flaviviruses evolved a unique strategy to generate a noncoding RNA product that allows them to kill the host more efficiently. |
| Databáze: | OpenAIRE |
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