A microfabricated fluidic device for performing two-dimensional liquid-phase separations
| Autor: | R. S. Ramsey, R. D. Rocklin, John Michael Ramsey |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chromatography
Miniaturization Resolution (mass spectrometry) Chemistry Analytical chemistry Electrophoresis Capillary Proteins Micellar electrokinetic chromatography Peptide Fragments Analytical Chemistry Electrophoresis Capillary electrophoresis Sampling (signal processing) Dimension (vector space) Fluidics Peptides Fluorescein-5-isothiocyanate Fluorescent Dyes |
| Zdroj: | Analytical chemistry. 72(21) |
| ISSN: | 0003-2700 |
| Popis: | A microfabricated fluidic device that combines micellar electrokinetic chromatography and high-speed open-channel electrophoresis on a single structure for the rapid automated two-dimensional analysis of peptides has been devised and demonstrated. The microchip operates by rapidly sampling and analyzing effluent in the second dimension from the first dimension. Second-dimension analyses are performed and completed every few seconds, with total analysis times of less than 10 min for tryptic peptides. The peak capacity of the two-dimensional separations has been estimated to be in the 500−1000 range. The orthogonality of the separation techniques, an important factor for maximizing peak capacity or resolution elements, was verified by examining each technique independently for peptide separations. The two-dimensional separation strategy was found to greatly increase the resolving power over that obtained for either dimension alone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|