Location of close contacts between Escherichia coli RNA polymerase and guanine residues at promoters either with or without consensus -35 region sequences
| Autor: | Steve Busby, Stephen D. Minchin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Guanine Transcription Genetic DNA Mutational Analysis Molecular Sequence Data lac operon medicine.disease_cause Methylation Biochemistry Structure-Activity Relationship chemistry.chemical_compound RNA polymerase Consensus Sequence Escherichia coli medicine Consensus sequence Promoter Regions Genetic Molecular Biology Base Composition Base Sequence biology Galactose Promoter DNA-Directed RNA Polymerases Cell Biology biology.organism_classification Molecular biology Enterobacteriaceae Lac Operon chemistry Research Article |
| Zdroj: | Biochemical Journal. 289:771-775 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | Methylation-interference assays have been used to identify guanine residues that make important contacts with RNA polymerase during open-complex formation at two related Escherichia coli promoters. Methylation of lower-strand G-31 at a gal consensus promoter completely prevents complex formation, while modification of upper-strand G-33 has no detectable effect. At galP1, which lacks a consensus -35 region, modification of lower-strand G-33 and upper-strand G-14 reduces, but does not prevent, complex formation. G-33 is the only guanine residue in the -35 region of galP1 where modification interferes with open-complex formation. Since this guanine residue is not protected in open complexes, we conclude that its modification causes alteration of, or interference with, a transient contact during the transcription initiation pathway. |
| Databáze: | OpenAIRE |
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