Multilocus Sequence Typing Reveals that the Population Structure of Candida dubliniensis is Significantly Less Divergent than that of Candida albicans
| Autor: | Marie-Elisabeth Bougnoux, Helena Bujdáková, Gary P. Moran, Silvia Borecka-Melkusova, Brenda A. McManus, Christophe d'Enfert, Philip Murphy, Emmanuelle Pinjon, David C. Coleman, Derek J. Sullivan, Dorothée Diogo |
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| Přispěvatelé: | Trinity College Dublin, Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Comenius University in Bratislava, Adelaide and Meath Hospital, Dublin Incorporating the National Children's Hospital, Partenaires INRAE, Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Genotype Population Sequence Homology Mycology Fungal Proteins 03 medical and health sciences Cluster Analysis Humans CANDIDA DUBLINIENSIS Internal transcribed spacer DNA Fungal Mycological Typing Techniques education Candida albicans Genotyping Candida DNA Primers 030304 developmental biology Genetics Molecular Epidemiology 0303 health sciences education.field_of_study Polymorphism Genetic biology 030306 microbiology GENOMIC Candidiasis Fungal genetics Sequence Analysis DNA biology.organism_classification [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Amino Acid Substitution DNA profiling CANDIDA ALBICANS Multilocus sequence typing MULTILOCUS SEQUENCE TYPING TYPING Candida dubliniensis |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2008, 46 (2), pp.652-664. ⟨10.1128/JCM.01574-07⟩ Journal of Clinical Microbiology, 2008, 46 (2), pp.652-664. ⟨10.1128/JCM.01574-07⟩ |
| ISSN: | 0095-1137 |
| Popis: | The pathogenic yeast Candida dubliniensis is phylogenetically very closely related to Candida albicans , and both species share many phenotypic and genetic characteristics. DNA fingerprinting using the species-specific probe Cd25 and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal gene cluster previously showed that C. dubliniensis is comprised of three major clades comprising four distinct ITS genotypes. Multilocus sequence typing (MLST) has been shown to be very useful for investigating the epidemiology and population biology of C. albicans and has identified many distinct major and minor clades. In the present study, we used MLST to investigate the population structure of C. dubliniensis for the first time. Combinations of 10 loci previously tested for MLST analysis of C. albicans were assessed for their discriminatory ability with 50 epidemiologically unrelated C. dubliniensis isolates from diverse geographic locations, including representative isolates from the previously identified three Cd25-defined major clades and the four ITS genotypes. Dendrograms created by using the unweighted pair group method with arithmetic averages that were generated using the data from all 10 loci revealed a population structure which supports that previously suggested by DNA fingerprinting and ITS genotyping. The MLST data revealed significantly less divergence within the C. dubliniensis population examined than within the C. albicans population. These findings show that MLST can be used as an informative alternative strategy for investigating the population structure of C. dubliniensis . On the basis of the highest number of genotypes per variable base, we recommend the following eight loci for MLST analysis of C. dubliniensis : Cd AAT1b , Cd ACC1 , Cd ADP1 , Cd MPIb , Cd RPN2 , Cd SYA1 , exCd VPS13 , and exCd ZWF1b , where “Cd” indicates C. dubliniensis and “ex” indicates extended sequence. |
| Databáze: | OpenAIRE |
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