An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens
| Autor: | Lynn M. Thomson, C. Doig, Lucia Garcia, Ken J. Forbes, Hamidou Traore, Herdis Steingrimsdottir, Hasan Yesilkaya |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Lysis Tuberculosis Detergents Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Mycobacterium tuberculosis chemistry.chemical_compound law medicine Humans Tuberculosis Pulmonary Molecular Biology Polymerase chain reaction biology Sputum biology.organism_classification medicine.disease genomic DNA chemistry Reagent Kits Diagnostic medicine.symptom DNA Mycobacterium |
| Zdroj: | Journal of Microbiological Methods. 63:95-98 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2005.03.005 |
| Popis: | A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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