Mash1 efficiently reprograms rat astrocytes into neurons
| Autor: | Daofang Ding, Hao Xu, Yongjun Wang, Yongjian Zhao, Qianqian Liang, Xiao-Feng Li, Leqin Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Research and Report
neurons Brn2 law.invention 03 medical and health sciences 0302 clinical medicine Plasmid Retrovirus Developmental Neuroscience law plasmid the National Basic Research Program of China (973 Program) reprogram nerve regeneration 030304 developmental biology 0303 health sciences biology astrocytes Transfection biology.organism_classification 3. Good health retrovirus Recombinant DNA β-tubulin Neural regeneration MASH-1 neural regeneration Neuroscience 030217 neurology & neurosurgery Mash-1 |
| Zdroj: | Neural Regeneration Research |
| ISSN: | 1876-7958 1673-5374 |
| Popis: | To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mash1 and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mash1 was not detectable. Thus, we hypothesized that Mash1 alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mash1. One week later, we observed the changes in morphology of astrocytes, which showed typical neuronal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mash1 than in control cells. These results indicate that Mash1 alone can reprogram astrocytes into neurons. |
| Databáze: | OpenAIRE |
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