Measurement of the relative non-degenerate two-photon absorption cross-section for fluorescence microscopy
| Autor: | Anna Devor, Payam A. Saisan, Sanaz Sadegh, Christopher G. L. Ferri, Yeshaiahu Fainman, Martin Thunemann, Mu-Han Yang |
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| Rok vydání: | 2020 |
| Předmět: |
Physics
Photon Fluorophore business.industry Physics::Optics 02 engineering and technology Photon energy 021001 nanoscience & nanotechnology 01 natural sciences Molecular physics Two-photon absorption Fluorescence Atomic and Molecular Physics and Optics Fluorescence spectroscopy Article 010309 optics chemistry.chemical_compound Optics chemistry 0103 physical sciences Microscopy 0210 nano-technology business Excitation |
| Zdroj: | Optics express. 27(6) |
| ISSN: | 1094-4087 |
| Popis: | In non-degenerate two-photon microscopy (ND-TPM), the required energy for fluorescence excitation occurs via absorption of two photons of different energies derived from two synchronized pulsed laser beams. ND-TPM is a promising imaging technology offering flexibility in the choice of the photon energy for each beam. However, a formalism to quantify the efficiency of two-photon absorption (TPA) under non-degenerate excitation, relative to the resonant degenerate excitation, is missing. Here, we derive this formalism and experimentally validate our prediction for a common fluorophore, fluorescein. An accurate quantification of non-degenerate TPA is important to optimize the choice of photon energies for each fluorophore. |
| Databáze: | OpenAIRE |
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