The Relevance of Vascular Endothelial Growth Factor, Hypoxia Inducible Factor-1 Alpha, and Clusterin in Carotid Plaque Instability

Autor: Afshin Assadian, Nikolaus Duschek, Jürgen Falkensammer, Sabine Rauscher, Christoph Kaun, Stefan Stojkovic, Jelena Basic, Johann Wojta
Rok vydání: 2018
Předmět:
Male
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
medicine.medical_treatment
Carotid endarterectomy
Asymptomatic
Severity of Illness Index
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine.artery
medicine
Humans
Carotid Stenosis
Prospective Studies
RNA
Messenger
Aged
Aged
80 and over
Clusterin
biology
Neovascularization
Pathologic
Rupture
Spontaneous
business.industry
Rehabilitation
Hypoxia (medical)
Middle Aged
medicine.disease
Hypoxia-Inducible Factor 1
alpha Subunit
Plaque
Atherosclerotic
Vascular endothelial growth factor
Stroke
Stenosis
chemistry
Gene Expression Regulation
Asymptomatic Diseases
biology.protein
Immunohistochemistry
Surgery
Female
Neurology (clinical)
Internal carotid artery
medicine.symptom
Cardiology and Cardiovascular Medicine
business
030217 neurology & neurosurgery
Carotid Artery
Internal
Zdroj: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association. 28(6)
ISSN: 1532-8511
Popis: Background: Stroke is one of the leading causes of morbidity and mortality. Thromboembolism, as a major cause of carotid artery-related stroke, can be caused by plaque rupture which is associated with neoangiogenesis within the carotid plaque. Aim: We sought to investigate a possible correlation between angiogenesis-related factors and preoperative neurological manifestations in patients with internal carotid artery stenosis, for a better understanding of thromboembolism in internal carotid artery stenosis-related stroke. Methods: This study included 54 patients (asymptomatic, n = 20 and symptomatic, n = 34) undergoing carotid endarterectomy for high-grade internal carotid artery stenosis. In the retrieved carotid plaques, angiogenesis-related factors (vascular endothelial growth factor [VEGF], hypoxia inducible factor-1 alpha [HIF-1α], and Clusterin) were measured by immunohistochemistry and quantified by real-time polymerase chain reaction. Results: We demonstrated the expression of VEGF, HIF-1α, and Clusterin by endothelial cells and smooth muscle cells in the carotid plaques. Noteworthy, mRNA VEGF levels were .7-fold higher in symptomatic patients (P = .017) compared to asymptomatic patients. In contrast, mRNA Clusterin levels were 1.8-fold lower (P = .021). Levels of mRNA HIF-1α were 1.5-fold higher in asymptomatic patients, but no statistical significance was reached between the 2 groups. Conclusions: Our results show an association between VEGF and Clusterin and neurological symptoms of patients with high-grade carotid artery stenosis.
Databáze: OpenAIRE
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