batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor
| Autor: | Sophie Netter, Laurent Théodore, M. Faucheux, Christophe Antoniewski, J. Charollais, Jean-Yves Roignant |
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| Rok vydání: | 2003 |
| Předmět: |
Embryo
Nonmammalian Protein family Molecular Sequence Data Repressor Response Elements Chromosomes Conserved sequence Animals Drosophila Proteins Amino Acid Sequence Molecular Biology Gene Transcription factor Psychological repression Conserved Sequence Transcriptional Regulation Homeodomain Proteins Polycomb Repressive Complex 1 Genetics Base Sequence biology fungi Gene Expression Regulation Developmental Cell Biology biology.organism_classification Protein Structure Tertiary DNA-Binding Proteins Repressor Proteins Drosophila melanogaster Larva Homeotic gene Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 23:1181-1195 |
| ISSN: | 1098-5549 |
| Popis: | Polycomb and trithorax group genes maintain the appropriate repressed or activated state of homeotic gene expression throughout Drosophila melanogaster development. We have previously identified the batman gene as a Polycomb group candidate since its function is necessary for the repression of Sex combs reduced. However, our present genetic analysis indicates functions of batman in both activation and repression of homeotic genes. The 127-amino-acid Batman protein is almost reduced to a BTB/POZ domain, an evolutionary conserved protein-protein interaction domain found in a large protein family. We show that this domain is involved in the interaction between Batman and the DNA binding GAGA factor encoded by the Trithorax-like gene. The GAGA factor and Batman codistribute on polytene chromosomes, coimmunoprecipitate from nuclear embryonic and larval extracts, and interact in the yeast two-hybrid assay. Batman, together with the GAGA factor, binds to MHS-70, a 70-bp fragment of the bithoraxoid Polycomb response element. This binding, like that of the GAGA factor, requires the presence of d(GA)n sequences. Together, our results suggest that batman belongs to a subset of the Polycomb/trithorax group of genes that includes Trithorax-like, whose products are involved in both activation and repression of homeotic genes. |
| Databáze: | OpenAIRE |
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